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哮喘小鼠模型
分类: 呼吸
发布时间: 2020-03-19 00:00
方法:明矾和OVA致敏的哮喘
品系:Balb/c小鼠,SPF级,雄性,周龄:4-6周,体重:20g-22g
类型:成品模型
名称:Mouse Model for Asthma
编号:LM80232
品系:Balb/c小鼠,SPF级,雄性,周龄:4-6周,体重:20g-22g
类型:成品模型
名称:Mouse Model for Asthma
编号:LM80232
模型评价
1.一般观察 哮喘模型组小鼠在激发开始后出现打喷嚏、点头呼吸、呼吸急促、哮鸣音、腹肌收缩、烦躁等典型哮喘发作症状,并且后期观察中发现饮食减少、毛色无光泽、行动迟缓及体重逐渐下降。
2.支气管肺泡灌洗液(BALF)的收集最后1次激发24 h后(第28天)将各组小鼠颈椎脱臼处死,无菌条件下分离气管,结扎左主支气管后,剪开右主支气管插入气管导管并固定,无菌生理盐水灌洗3次,1mL/次,收集的BALF于4℃,1200 R/min离心5 min,弃去上清液,PBS重悬细胞沉淀并涂片,进行细胞分类。
1.一般观察 哮喘模型组小鼠在激发开始后出现打喷嚏、点头呼吸、呼吸急促、哮鸣音、腹肌收缩、烦躁等典型哮喘发作症状,并且后期观察中发现饮食减少、毛色无光泽、行动迟缓及体重逐渐下降。
2.支气管肺泡灌洗液(BALF)的收集最后1次激发24 h后(第28天)将各组小鼠颈椎脱臼处死,无菌条件下分离气管,结扎左主支气管后,剪开右主支气管插入气管导管并固定,无菌生理盐水灌洗3次,1mL/次,收集的BALF于4℃,1200 R/min离心5 min,弃去上清液,PBS重悬细胞沉淀并涂片,进行细胞分类。
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